Topic > Characteristics of Metroxylon Sagu - 686

INTRODUCTION Metroxylon sagu (M. sagu) or sago palm is one of the plants capable of producing starch. Starch production from M. sagu is four times higher than that from Oryza sativa or rice (Lal, 2003). M. sagu is also able to resist stress and not many molecular studies have been reported regarding this plant. The characteristics of M. sagu can be studied by carrying out a transcriptome analysis which can better explain the development of the plant starting from gene expression. In the article I had chosen, "Expressed sequence tags (ESTs) from young leaves of Metroxylon sagu" by Ching Ching Wee and Hairul Azman Roslan, they had constructed a cDNA library and a preliminary analysis of sequence tags expressed from young leaves of M. sagu . From the results, 434 of the total clones were sequenced with inserts ranging from 1,000 to 3,000 bps with primary and amplified titers of 8 9 105 and 1.0 9 109 pfu/ml, respectively. Clustering these sequences shows that in a set of 372 provisional unigenes, there are 340 singletons and 32 contigs. The BLAST2GO annotated database showed that most transcripts are involved in stress tolerance and primary metabolism. To gain a better understanding of the gene expression and molecular mechanism of M. sagu development and its ability to resist stress, an expressed sequence tag (EST) library had been conducted. The effective tool for gene expression research is the cDNA library. He created the expression pattern from the selected fabric which in this case used young leaves. The presence of mRNA in the selected tissue and sampling conditions will be represented by the ESTs that have been generated. The EST database that had been built had some advantages that… halfway through the paper… sequence analysis shows that tentive unique genes (TUG) sequences yielded 372 contiguous sequences and 340 singletons. This library has a redundancy of *17% and a GC level of 45.02%. For functional annotation of EST sequence results, BLASTx analysis shows that 86.56% of TUGs had high homology to known proteins while 8.06% of TUGs had low homology to known proteins. A total of 20 TUGs do not match any proteins in the database, so they may hold the potential to have the new function in M. sagu. A total of 1,710 GO terms were assigned from the annotated sequences. They have been classified into three main GO categories: molecular function (960), cellular component (2, 384), and biological process (2, 156). Some GO terms were mapped into one or more GO categories and that is why the total number of these three GOs was higher 1, 710.